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Labchart 8 Labchart Pro Upgrade V8 For Windows, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ADInstruments in vivo plethysmograph recording labchart pro v 8.0.10
Respiratory activity was recorded in vivo in a two-chamber <t>plethysmograph</t> ( a ). Sighs, defined by an increase in inspiratory volume and respiratory cycle period with a biphasic inspiration ( b ), increase in frequency after intracerebroventricular injection (i.c.v.) of PGE 2 . This effect is absent in mice lacking EP3R ( Ptger3 -/- , c , arrows, d ). I.c.v. injection of PGE 2 also increases the tidal volume (V T ) in wild-type C57BL/6J (WT) mice ( e ). The sigh frequency is increased by hypercapnic (5% CO 2 in normoxia) conditions in wild-type and Ptger3 -/- mice but less so in Ptger3 -/- mice ( f ). In wild-type mice, the increase is abolished after i.c.v. injection of PGE 2 ( f ). Hypercapnic exposure causes an increase in respiratory frequency (F R ), tidal volume (V T ), and minute ventilation (V E ) ( g ), but the increase is attenuated in Ptger3 -/- mice. Data are presented as means ± SD. *p<0.05 Source data are available in a separate source data file. DOI: http://dx.doi.org/10.7554/eLife.14170.004 10.7554/eLife.14170.005 Figure 1—source data 1. In vivo plethysmography data. DOI: http://dx.doi.org/10.7554/eLife.14170.005
In Vivo Plethysmograph Recording Labchart Pro V 8.0.10, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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POWERLAB INC labchart pro v.8
Respiratory activity was recorded in vivo in a two-chamber <t>plethysmograph</t> ( a ). Sighs, defined by an increase in inspiratory volume and respiratory cycle period with a biphasic inspiration ( b ), increase in frequency after intracerebroventricular injection (i.c.v.) of PGE 2 . This effect is absent in mice lacking EP3R ( Ptger3 -/- , c , arrows, d ). I.c.v. injection of PGE 2 also increases the tidal volume (V T ) in wild-type C57BL/6J (WT) mice ( e ). The sigh frequency is increased by hypercapnic (5% CO 2 in normoxia) conditions in wild-type and Ptger3 -/- mice but less so in Ptger3 -/- mice ( f ). In wild-type mice, the increase is abolished after i.c.v. injection of PGE 2 ( f ). Hypercapnic exposure causes an increase in respiratory frequency (F R ), tidal volume (V T ), and minute ventilation (V E ) ( g ), but the increase is attenuated in Ptger3 -/- mice. Data are presented as means ± SD. *p<0.05 Source data are available in a separate source data file. DOI: http://dx.doi.org/10.7554/eLife.14170.004 10.7554/eLife.14170.005 Figure 1—source data 1. In vivo plethysmography data. DOI: http://dx.doi.org/10.7554/eLife.14170.005
Labchart Pro V.8, supplied by POWERLAB INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Respiratory activity was recorded in vivo in a two-chamber plethysmograph ( a ). Sighs, defined by an increase in inspiratory volume and respiratory cycle period with a biphasic inspiration ( b ), increase in frequency after intracerebroventricular injection (i.c.v.) of PGE 2 . This effect is absent in mice lacking EP3R ( Ptger3 -/- , c , arrows, d ). I.c.v. injection of PGE 2 also increases the tidal volume (V T ) in wild-type C57BL/6J (WT) mice ( e ). The sigh frequency is increased by hypercapnic (5% CO 2 in normoxia) conditions in wild-type and Ptger3 -/- mice but less so in Ptger3 -/- mice ( f ). In wild-type mice, the increase is abolished after i.c.v. injection of PGE 2 ( f ). Hypercapnic exposure causes an increase in respiratory frequency (F R ), tidal volume (V T ), and minute ventilation (V E ) ( g ), but the increase is attenuated in Ptger3 -/- mice. Data are presented as means ± SD. *p<0.05 Source data are available in a separate source data file. DOI: http://dx.doi.org/10.7554/eLife.14170.004 10.7554/eLife.14170.005 Figure 1—source data 1. In vivo plethysmography data. DOI: http://dx.doi.org/10.7554/eLife.14170.005

Journal: eLife

Article Title: CO 2 -evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture

doi: 10.7554/eLife.14170

Figure Lengend Snippet: Respiratory activity was recorded in vivo in a two-chamber plethysmograph ( a ). Sighs, defined by an increase in inspiratory volume and respiratory cycle period with a biphasic inspiration ( b ), increase in frequency after intracerebroventricular injection (i.c.v.) of PGE 2 . This effect is absent in mice lacking EP3R ( Ptger3 -/- , c , arrows, d ). I.c.v. injection of PGE 2 also increases the tidal volume (V T ) in wild-type C57BL/6J (WT) mice ( e ). The sigh frequency is increased by hypercapnic (5% CO 2 in normoxia) conditions in wild-type and Ptger3 -/- mice but less so in Ptger3 -/- mice ( f ). In wild-type mice, the increase is abolished after i.c.v. injection of PGE 2 ( f ). Hypercapnic exposure causes an increase in respiratory frequency (F R ), tidal volume (V T ), and minute ventilation (V E ) ( g ), but the increase is attenuated in Ptger3 -/- mice. Data are presented as means ± SD. *p<0.05 Source data are available in a separate source data file. DOI: http://dx.doi.org/10.7554/eLife.14170.004 10.7554/eLife.14170.005 Figure 1—source data 1. In vivo plethysmography data. DOI: http://dx.doi.org/10.7554/eLife.14170.005

Article Snippet: From in vivo plethysmograph recording (LabChart Pro, v 8.0.10, AD Instruments, Dunedin, New Zealand), periods of calm respiration without movement artifacts were selected for analysis based upon visual observations during experimentation as in previous studies ( ).

Techniques: Activity Assay, In Vivo, Injection