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Image Search Results
Journal: eLife
Article Title: CO 2 -evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture
doi: 10.7554/eLife.14170
Figure Lengend Snippet: Respiratory activity was recorded in vivo in a two-chamber plethysmograph ( a ). Sighs, defined by an increase in inspiratory volume and respiratory cycle period with a biphasic inspiration ( b ), increase in frequency after intracerebroventricular injection (i.c.v.) of PGE 2 . This effect is absent in mice lacking EP3R ( Ptger3 -/- , c , arrows, d ). I.c.v. injection of PGE 2 also increases the tidal volume (V T ) in wild-type C57BL/6J (WT) mice ( e ). The sigh frequency is increased by hypercapnic (5% CO 2 in normoxia) conditions in wild-type and Ptger3 -/- mice but less so in Ptger3 -/- mice ( f ). In wild-type mice, the increase is abolished after i.c.v. injection of PGE 2 ( f ). Hypercapnic exposure causes an increase in respiratory frequency (F R ), tidal volume (V T ), and minute ventilation (V E ) ( g ), but the increase is attenuated in Ptger3 -/- mice. Data are presented as means ± SD. *p<0.05 Source data are available in a separate source data file. DOI: http://dx.doi.org/10.7554/eLife.14170.004 10.7554/eLife.14170.005 Figure 1—source data 1. In vivo plethysmography data. DOI: http://dx.doi.org/10.7554/eLife.14170.005
Article Snippet: From in
Techniques: Activity Assay, In Vivo, Injection